ABSTRACT
Background: The prevalence of nosocomial infections has continued to rise due to microbial contamination of the hospital environment. Operating room contamination, on the other hand, is one of the most common and life-threatening sources of nosocomial infections. Objective: The goal of this research is to isolate and identify bacterial contaminants in operating rooms and equipment in EnNahud, West Kordofan. Methods: A total of 45 samples (from three hospitals) were collected from various operating theatre sites between September and December 2020. Using the accepted bacteriological methods (ISO/TC 147/SC 4 Microbiological techniques), all isolated bacteria were identified. S. Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 were employed as control bacterial strains to track the entire bacteriological process. Results: Five kinds of bacteria were recovered from the 45 (100%) positive specimens in three hospitals in this study. The number of polluted hospitals is on the rise (100%). The most prevalent bacterial pollutants identified from operation theaters were Staphylococcus aureus 24 (53.3%), Pseudomonas aeruginosa 13 (28.8%), Bacillus spp 6 (13.3%), Proteus spp 1 (2.2%), and Salmonella spp 1 (2.2%), according to the findings (2.2%). All of the places where samples were taken were found to be completely contaminated. Conclusion: The relatively high degree of bacterial contamination in hospital operating rooms highlights the need for ongoing microbiological surveillance aimed at detecting bacterial contamination levels and their impact on nosocomial infection early.